Phosphatidylinositol-phospholipase C4 suppresses the hypersensitive response of <i>Nicotiana benthamiana</i>

Phospholipid signaling plays an important role in plant immune responses. Here, we isolated two phospholipase C4 (PLC4) orthologs the Nicotiana benthamiana genome, designated as N. PLC4-1 and PLC4-2 (NbPLC4-1 NbPLC4-2). We created NbPLC4-1- NbPLC4-2- silenced plants. Induction of hypersensitive response (HR), including HR cell death bacterial population reduction, was accelerated both NbPLC4-2-silenced plants challenged with benthamiana-incompatible Ralstonia solanacearum 8107. The also showed enhanced expression Nbhin1, a marker gene. Expressions genes for salicylic acid (SA) jasmonic (JA) were drastically increased by R. inoculation. In addition, NbPLC4-1 NbPLC4-2 silencing triggered reactive oxygen species (ROS) hyper-production. These results suggest that NbPLC4s are closely associated JA, SA, ROS act negative regulators benthamiana.